UMA
Master en Biotecnologia Avanzada
Bioinformatica y Tratamiento de Datos (BIF)
2025

Redes Biologicas y Teoría de Grafos

CytoScape 3.10.x practical


  • Download and install latest version of Cytoscape: http://cytoscape.org/download.php

  • Get familiar with main Cytoscape file formats (->)

  • Load a "cytoscape sesion" (.cys) file (network plus nodes' and edges' associated data): File > Open > galFiltered.cys (directory: sampleData)

  • Cytoscape panels (network panel, table panel, control panel)

  • Navigate (zoom in/out, scale, move, ...)

  • Try a couple of different layouts.

  • Select/unselect a node. Inspect its properties on the "Data Panel". Linkout.

  • Select nodes by their names in the "search bar".

  • Sort "Data Panel" (e.g. by "degree") and select nodes there.

  • Move a node; select the first neighbors of a node and move them together; ...

  • Select a bunch of nodes/edges. Move them together, and inspect their properties on the "Data Panel"

  • Style tab: Try a couple of different styles for visualizing the network.

  • Style: Play with the "Default" parameters for the representation ("Style > Def"): e.g. node shape, node border width and color, edge width, ...

  • Change repersentation for subset of selected nodes ("Style > Byp")

  • Associate graphical elements to network parameters ("mapping") ("Style > Map."):

  • Node size proportional to Degree. Node color representing "betweennessCentrality" in a color scale.

  • Edge width proportional to "edge betweenness". Add edge arrow for protein-DNA interactions ("pd", interactions representing transcriptional control).

  • Select a bunch of nodes. "Propagate" them by heat diffusion: Tools > Diffuse > Selected nodes with options

  • Color nodes by propagated heat.

  • Save session / Export image, ...

  • Import this network from a external text file: test_net.tsv (File > Import > Network from file). And this file with attributes for the nodes: test_net_attr.tsv (File > Import > Table from file).

  • Take a look at APPs.

Additional resources

Florencio Pazos Cabaleiro
pazos@cnb.csic.es
http://csbg.cnb.csic.es
Tlf. +34.91.5854669